Human PP14 (Placental Protein 14) ELISA Kit
Catalog Number: ACE-E1704
Size: 96 tests/kit
Detection Range: 4.69—300 ng/mL
Sensitivity: 2.81 ng/mL
Datasheet
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Size: 96 tests/kit
Detection Range: 4.69—300 ng/mL
Sensitivity: 2.81 ng/mL
Datasheet
Request Information
Price on request
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You are enquiring about Human PP14 (Placental Protein 14) ELISA Kit
Description:
This ELISA kit applies to the in vitro quantitative determination of Human PP14 concentrations in serum, plasma and other biological fluids.
Assay Principles: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PP14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PP14. You can calculate the concentration of Human PP14 in the samples by comparing the OD of the samples to the standard curve.
Species reactivity:
Human
Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
This ELISA kit applies to the in vitro quantitative determination of Human PP14 concentrations in serum, plasma and other biological fluids.
Assay Principles: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PP14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PP14. You can calculate the concentration of Human PP14 in the samples by comparing the OD of the samples to the standard curve.
Species reactivity:
Human
Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
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