Human PRSS50 (Protease, Serine 50) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 50(PRSS50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 50(PRSS50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 50(PRSS50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease, Serine 50(PRSS50) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
TSP50, CT20, Cancer/Testis Antigen 20, Testes Specific Protease 50, Testis-specific protease-like protein 50

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only