Human PTX3 (Pentraxin 3, Long) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pentraxin 3, Long(PTX3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pentraxin 3, Long(PTX3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pentraxin 3, Long(PTX3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pentraxin 3, Long(PTX3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Tumor immunity

Alternative Names:
TNFAIP5, TSG-14, Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta, Tumor Necrosis Factor Alpha-Induced Protein 5, Tumor necrosis factor-inducible gene 14 protein

Sample Type:
serum, plasma, tissue homogenates, cell lysates, salivas and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only