Human SAA (Serum Amyloid A) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Serum Amyloid A(SAA) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloid A(SAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid A(SAA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity, Kidney biomarker, Hepatology, Urology

Alternative Names:
SA-A, SAA1, PIG4, TP53I4, Amyloid protein A, Amyloid fibril protein AA, Serum amyloid A-1 protein

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Competitive Inhibition

Standard: 10000 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only