Human SARS-CoV-2 Nucleocapsid ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infectious Diseases

Alternative Names:
COVID-19；SARS-CoV-2； 2019-nCoV

Sample Type:
serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 500 pg/mL

Assay Length: 3.5h

Assay Length: For Research Use Only