Human SNAP23 (Synaptosomal-associated protein 23) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SNAP23(Synaptosomal-associated protein 23). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SNAP23(Synaptosomal-associated protein 23). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SNAP23(Synaptosomal-associated protein 23), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SNAP23(Synaptosomal-associated protein 23) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cancer

Alternative Names:
Synaptosomal-associated protein 23 , SNAP-23 , Vesicle-membrane fusion protein SNAP-23 , SNAP23

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only