Human SRI (Sorcin) ELISA Kit
€0.00
In stock
SKU
ELK5034
Catalog Number: ELK5034
Reactivity: Human
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.119 ng/mL
Information
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Reactivity: Human
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.119 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorcin(SRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorcin(SRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorcin(SRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorcin(SRI) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Signal transduction,
Alternative Names:
SCN, CP-22, V19, 22 kDa protein
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Standard: 20 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorcin(SRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorcin(SRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorcin(SRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorcin(SRI) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Signal transduction,
Alternative Names:
SCN, CP-22, V19, 22 kDa protein
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Standard: 20 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
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