Human STAB1 (Stabilin-1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to STAB1(Stabilin-1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to STAB1(Stabilin-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STAB1(Stabilin-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of STAB1(Stabilin-1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal Transduction, Epigenetics and Nuclear Signaling

Alternative Names:
STAB1 , Stabilin-1 , Fasciclin , EGF-like , laminin-type EGF-like and link domain-containing scavenger receptor 1 , FEEL-1 , MS-1 antigen , FEEL1 , KIAA0246 , FEX1 , FELE-1 , STAB-1 , CLEVER-1 , common lymphatic endothelial and vascular endothelial receptor-1

Sample Type:
serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only