Human TBX21 (T-Box Protein 21) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21(TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21(TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21(TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Box Protein 21(TBX21) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
TBLYM, TBET, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet

Sample Type:
Cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only