Human TCIRG1 (T-Cell, Immune Regulator 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1(TCIRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell, Immune Regulator 1(TCIRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell, Immune Regulator 1(TCIRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell, Immune Regulator 1(TCIRG1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity

Alternative Names:
Stv1, Vph1, ATP6N1C, ATP6V0A3, Atp6i, OC-116kDa, OC116, OPTB1, TIRC7, a3, V-Type Proton ATPase 116 kDa Subunit A Isoform 3, Osteoclastic proton pump 116 kDa subunit

Sample Type:
Serum and other biological fluids.

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only