Human Thrombomodulin ELISA Kit
€0.00
In stock
SKU
BEK1374
Catalog Number: BEK1374
Size(s): 96 tests/kit
Standard Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <27 pg/ml
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Size(s): 96 tests/kit
Standard Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <27 pg/ml
Request Information
Introduction:
Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme.Thrombomodulin is also expressed on human mesothelial cell,monocyte and a dendritic cell subset.In humans, thrombomodulin is encoded by the THBD gene.[7] The protein has a molecular mass of 74kDa, and consists of a single chain with six tandemly repeated EGF-like domains, a Serine/Threonine-rich spacer and a transmembrane domain.[8] It is a member of the C-type lectin domain (CTLD) group 14 family.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Thrombomodulin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Thrombomodulin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Thrombomodulin amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of Thrombomodulin can be calculated.
Application:
For quantitative detection of Thrombomodul in in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme.Thrombomodulin is also expressed on human mesothelial cell,monocyte and a dendritic cell subset.In humans, thrombomodulin is encoded by the THBD gene.[7] The protein has a molecular mass of 74kDa, and consists of a single chain with six tandemly repeated EGF-like domains, a Serine/Threonine-rich spacer and a transmembrane domain.[8] It is a member of the C-type lectin domain (CTLD) group 14 family.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Thrombomodulin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Thrombomodulin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Thrombomodulin amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of Thrombomodulin can be calculated.
Application:
For quantitative detection of Thrombomodul in in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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