Human thrombospondin 2 (TSP-2) ELISA Kit

INTENDED USE:
For the quantitative determination of human TSP-2 concentrations in serum or plasma samples

INTRODUCTION:
Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein. TSP-2 inhibits angiogenesis through direct effect on endothelial cell migration, proliferation, survival and apoptosis. It is a potential risk factor in heart disease.

PRINCIPLE OF THE ASSAY:
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay.

SENSITIVITY: The lowest insulin level that can be measured by this assay is 0.156 ng/mL.