Human tPK1 (Tau-Protein Kinase 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tau-Protein Kinase 1(tPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tau-Protein Kinase 1(tPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tau-Protein Kinase 1(tPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tau-Protein Kinase 1(tPK1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Enzyme & Kinase, Metabolic pathway, Tumor immunity, Neuro science, Genetic science, Hepatology, Gastroenterology,

Alternative Names:
TTBK1, BDTK, TTK, Cdk5/P20, CDK5/P23, GSK, STK31, Brain-derived tau kinase, Tau-Tubulin Kinase 1, Tau-Protein O-Hosphotransferase, Brain Protein Kinase PK40erk

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only