Human Tumor Necrosis Factor-beta, TNF-β HiLA Kit
€0.00
In stock
SKU
BHK1017
Introduction:
Lymphotoxin-alpha (LT-α) formerly known as tumor necrosis factor-beta (TNF-β) is a protein that in humans is encoded by the LTA gene. Belonging to the hematopoietic cell line, LT-α exhibits anti-proliferative activity and causes the cellular destruction of tumor cell lines. As a cytotoxic protein, LT-α performs a variety of important roles in immune regulation depending on the form that it is secreted as. Unlike other members of the TNF superfamily, LT-α is only found as a soluble homotrimer, when found at the cell surface it is found only as a heterotrimer with LTβ.
Principle of the Assay:
Incubate the Fconjugated anti-TNF-β antibody 1, biotin conjugated anti-TNF-β antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration of TNF-β in the sample is calculated through mathe matical fitting. In the absence of human TNF-β in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human TNF-β in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
Lymphotoxin-alpha (LT-α) formerly known as tumor necrosis factor-beta (TNF-β) is a protein that in humans is encoded by the LTA gene. Belonging to the hematopoietic cell line, LT-α exhibits anti-proliferative activity and causes the cellular destruction of tumor cell lines. As a cytotoxic protein, LT-α performs a variety of important roles in immune regulation depending on the form that it is secreted as. Unlike other members of the TNF superfamily, LT-α is only found as a soluble homotrimer, when found at the cell surface it is found only as a heterotrimer with LTβ.
Principle of the Assay:
Incubate the Fconjugated anti-TNF-β antibody 1, biotin conjugated anti-TNF-β antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration of TNF-β in the sample is calculated through mathe matical fitting. In the absence of human TNF-β in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human TNF-β in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
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