Human ULBP2 (UL16 Binding Brotein 2) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UL16 Binding Brotein 2(ULBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UL16 Binding Brotein 2(ULBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UL16 Binding Brotein 2(ULBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UL16 Binding Brotein 2(ULBP2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
N2DL2, RAET1H, NKG2D Ligand 2, Retinoic acid early transcript 1H, NKG2D ligand 2

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only