Human VEGF165 recombinant active protein

Background Information: Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Target Name: Vascular Endothelial Growth Factor Isoform 165; VEGF165, VEGFA, VEGF-165

Source: Recombinant Human VEGF165 (accession number P15692-4) (Ala27-Arg191) is expressed in HEK293 cells.

Molecular Weight:
The 165 amino acid protein has the predicted molecular weight of 19.1kDa. The protein migrates at approximately 18-22kDa on SDS-PAGE with DTT-reduced condition.

Bioactivity: Tested by binding assay.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.2.

Endotoxin level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage and Handling:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.