Huntingtin polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
The huntingtin gene, also called HTT or HD(Huntington disease) gene, is the IT15("interesting transcript 15") gene which codes for a protein called the huntingtin protein. It is mapped to 4p16.3. The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents. HTT upregulates the expression of Brain Derived Neurotrophic Factor(BDNF) at the transcription level, and this gene is required for normal development. The HTT protein is involved in vesicle trafficking as it interacts with HIP1, a clathrin-binding protein, to mediate endocytosis, the absorption of materials into a cell. HTT was also visualized as punctate staining likely to represent nerve endings. What's more, wildtype HTT may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing.

Description:
Rabbit IgG polyclonal antibody for Huntingtin (HTT) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
AI256365 antibody, C430023I11Rik antibody, HD antibody, HD protein antibody, HDH antibody, HTT antibody, Huntingtin antibody, HUNTINGTON CHOREA antibody, Huntington disease protein antibody, IT 15 antibody, IT15 antibody, OTTMUSP00000026909 antibody, ZHD antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Huntingtin(3106-3121aa ELDRRAFQSVLEVVAA), different from the related mouse and rat sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.