IBA1 polyclonal, anti-human, mouse, rat

Background:
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

Alternative Name:
AIF-1, AIF1, G1, IBA1, AIF 1, AIF-1, Aif1, AIF1 protein, AIF1, Allograft inflammatory factor 1, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, IBA1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, Ionized calcium-binding adapter molecule 1, ionized calcium-binding adapter molecule, IRT 1, IRT1, Microglia response factor, MRF1, Protein g1,

Application Dilution:
WB:1:500
ICC/IF:1:50-1:100
IHC:1:100-1:500
FC:1:50-1:200
IP:1:10-1:50

Specificity: IBA1 polyclonal antibody detects endogenous levels of IBA1 protein.

Immunogen:
Recombinant protein

MW: 17 kDa

Swiss-Prot: P55008(Human) O70200(Mouse) P55009(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°Cafter thawing. Aliquot store at -20°Cor -80°CAvoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.