IBSP Polyclonal Antibody

Background:
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Research Area: Cancer, Signal Transduction, Stem Cells

Synonyms:
BNSP, Bone sialoprotein 2, Bone sialoprotein II, BSP, BSP II, BSPII, Cell binding sialoprotein, Cell-binding sialoprotein, IBSP, Integrin binding sialoprotein, Integrin-binding sialoprotein, SIAL, SPII

Immunogen: Synthetic peptide of human IBSP

Swissprot: P21815
Gene Accession: NP004958

Calculated MW: 35 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.84 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.