IDO (clone ID 177), anti-human
€204.00
In stock
SKU
ARP-23-A0035
Catalog Number: 23-A0035
Size: 25 µg
Isotype: Mouse IgG1 kappa
Size: 25 µg
Isotype: Mouse IgG1 kappa
Decription:
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Synonyms: Indoleamine 2,3-dioxygenase, INDO
Clone: ID 177
Isotype: Mouse IgG1 kappa
Immunogen: Recombinant human IDO.
Reactivity: human
Specificity: Recognizes human IDO. Detects a band of ~45kDa by Western blot. Other species not tested.
Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Applications:
ELISA: (direct or indirect: 1:2'000-1:10'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Working Dilution: Optimal conditions must be determined individually for each application.
Concentration: 1mg/ml
Storage: 2-8C immediate use, or -20C (aliquote)
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Synonyms: Indoleamine 2,3-dioxygenase, INDO
Clone: ID 177
Isotype: Mouse IgG1 kappa
Immunogen: Recombinant human IDO.
Reactivity: human
Specificity: Recognizes human IDO. Detects a band of ~45kDa by Western blot. Other species not tested.
Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Applications:
ELISA: (direct or indirect: 1:2'000-1:10'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Working Dilution: Optimal conditions must be determined individually for each application.
Concentration: 1mg/ml
Storage: 2-8C immediate use, or -20C (aliquote)
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