IFN-alpha 1 (clone 2-48), anti-human

Source:
A BALB/c mouse was immunized with E.coli derived recombinant human interferon α1.
Fusion partner: NS-0.

Specifications:
The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFNαfamily has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFNαare lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFNαinclude cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFNαare now known and seem to be expressed on most cell types. 2-48 Is specific for human interferon α1 and does not cross react with human interferon α2.

Species reactivity:
Positive: human.

Applications:
2-48 Can be used for the detection of human interferon α1 in ELISA and Western blot. It can be paired with interferon α1 mAb 2-52 to form an EIA to measure interferon α1.
ELISA: +
Frozen sections: +
Pair: +
Western blot: +

Positive control: Human interferon α 1.

References:
- Kontsek, P. et al.Mol Immunol. 29: 863-870 (1992)