IFN-γ polyclonal, anti-rat
€0.00
In stock
SKU
AC-ABG10166
Catalog Number: AC-ABG10166
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IFN-gamma. Anti-Rat IFN-gamma specific antibody was purified by affinity chromatography employing immobilized Rat IFN-gamma matrix.
Western Blot:
To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect Rat IFN-gamma by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat IFN-gamma (62-061BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.
Immunohistochemistry:
This antibody stained colchicine injected rat brain (including the cortex and median eminence) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IFN-gamma. Anti-Rat IFN-gamma specific antibody was purified by affinity chromatography employing immobilized Rat IFN-gamma matrix.
Western Blot:
To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect Rat IFN-gamma by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat IFN-gamma (62-061BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.
Immunohistochemistry:
This antibody stained colchicine injected rat brain (including the cortex and median eminence) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
| Is Featured? | No |
|---|
Write Your Own Review