Ig light chains kappa and lambda polyclonal, anti-rabbit

Quantity: 200 µl

Background:
As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rabbit serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types; also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As precipitating antiserum in a variety of immunodiffusion techniques including immunoelectrophoresis and double and single radial immunodiffusion Mancini, Ouchterlony). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Isotype: Swine Polyclonal

Source: Purified light chains isolated from polyclonal rabbit IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: rabbit

Cross Reactivity:
As tested in immunoelectrophoresis and double radial immunodiffusion anti pooled normal Rabbit Serum and purified IgG fractions and light chain prepaRations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types; also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in Rabbit Serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.