Ig light chains kappa and lambda polyclonal, anti-rat

Quantity: 200 µl

Background:
As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types; also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As precipitating antiserum in a variety of immunodiffusion techniques including immunoelectrophoresis and double and single radial immunodiffusion Mancini, Ouchterlony). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Isotype: Goat Polyclonal

Source: Purified light chains isolated from polyclonal rat IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Rat light chains, free and bound.

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but a wide scale of reactivity with IgG of other mammalian species must be assumed. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentRations of the reactants and the sensitivity of the assay format.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.