IgA (Fc specific) immunofixation polyclonal, anti-human

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. This antiserum is intended to detect and identify IgA in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Isotype: Goat Polyclonal

Source: Highly purified polyclonal IgA and monoclonal IgA2 isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Goat Ig, in phosphate buffered saline (PBS, pH 7.2).

Specificity:
Ig fraction of polyclonal Goat anti-Serum to Human immunoglobulin IgA, Fc specific

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.