IgA (Fc specific) polyclonal, anti-canine

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal dog serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in dog serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgA from serum or plasma. This antiserum does not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA.

Isotype: Rabbit Polyclonal

Source: Highly purified normal IgA isolated from pooled dog serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: Canine

Cross Reactivity:
The antiserum does not cross-react with any other component of the Dog Ig system. Interspecies cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.