IgD (Fc specific) polyclonal, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to the Fc portion of the IgD molecule, which represents strict isotype (class specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum belonging to different allotypic groups, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with the purified IgD from Balb/C and C57Bl. No precipitation reaction is obtained with purified IgG, including all subclasses, IgA, IgM, IgG/Fab fragments or other serum proteins. The antiserum also reacts with membranebound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgD in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgD from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.

Isotype: Sheep Polyclonal

Source: Highly purified polyclonal and monoclonal IgD isolated from Balb/C and C57Bl serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: mouse

Cross Reactivity:
The antiserum does not cross-react with any other component of the Mouse Ig system. Inter-species cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants, but this has not been tested because of the very low level of IgD in most species.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.