IgE (class specific) (clone NI 307), anti-human

Background:
The reactivity of the antiserum is restricted to the IgE molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions.

Clone: NI 307

Isotype: Mouse IgG1

Source: Highly purified monoclonal IgE isolated from human serum

Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Monoclonal Mouse antibody to Human IgE, isotype specific

Form: Delipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration is 1 mg/ml. No foreign proteins added.

Applications: Indirect immunofluorescence, ELISA

Storage: Store at 4C, or in small aliquots at -20C.