IgE (Fc specific) polyclonal, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to the Fc part of the IgE molecule, which represents strict isotype (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the mmunogen a single precipitin line is obtained which shows a reaction of full identity with the precipitin lines obtained against rat serum and ascites containing monoclonal IgE. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in rat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Isotype: Goat Polyclonal

Source: Highly purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Rat IgE, Fc specific

Reactivity: rat

Cross Reactivity:
The antiserum does not cross-react with any other component of the Rat immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, because of the very low level of IgE in most species.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.