IgE (Fc specific) polyclonal TRITC, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule as tested by gelimmunodiffusion techniques against rat sera and a panel of purified homogenous immunoglobulins. A reaction of full identity is obtained with serum containing monoclonal IgE and with the immunogen. No reaction is obtained with normal rat serum. The product does not react with any other protein of rat plasma or serum. To identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates; to identify circulating IgE antibodies in rat serum or other body fluids.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:10 and 1:60.

Isotype: Goat Polyclonal

Source: Purified homogenous IgE isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: TRITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Tetramethylrhodamine isothiocyanate–conjugated IgG fraction of polyclonal Goat anti-Serum to Rat IgE, Fc specific.

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.