IGFBP-2 mouse recombinant protein

Background:
IGFBP-2, also known as insulin-like growth factor binding protein 2, is a member of IGFBP superfamily. IGFBPs modulate the biological activities of IGF proteins and have a high affinity for IGFs. IGFBPs are not merely carrier proteins for IGFs, but hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues.This protein binds preferentially to IGF-2, exhibiting a 2 - 10 fold higher affinity for IGF-2 than for IGF-1. Also, it is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to the potential involvement of IGFBP-2 in tumorigenesis. Recombinant mouse IGFBP-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names:
insulin-like growth factor binding protein 2 isoform 1,insulin-like growth factor binding protein 2, IBP-2, Igfbp-2, mIGFBP-2, IGF-binding protein 2, Igfbp2

Domain: 35-305aa
Sequence:
EVLFRCPPCT PERLAACGPP PDAPCAELVR EPGCGCCSVC ARQEGEACGV YIPRCAQTLR CYPNPGSELP LKALVTGAGT CEKRRVGTTP QQVADSDDDH SEGGLVENHV DGTMNMLGGG SSAGRKPLKS GMKELAVFRE KVNEQHRQMG KGAKHLSLEE PKKLRPPPAR TPCQQELDQV LERISTMRLP DDRGPLEHLY SLHIPNCDKH GRYNLKQCKM SLNGQRGECW CVNPNTGKPI QGAPTIRGDP ECHLFYNEQQ ETGGAHAQSV Q

UniProt No.: P47877
NCBI Accession No.: NP_032368.2

Molecular Weight: 30.3kDa (277aa)
Concentration: 1mg/ml (determined by Absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 85% by SDS-PAGE
Bioactivity: Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Mouse IGF-2. The ED50 range ≤ 0.7 ug/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.