IgG (Fc specific) polyclonal, anti-equine

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal horse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in horse serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgG from serum or plasma.

Isotype: Goat Polyclonal

Source: Highly purified normal IgG isolated from pooled horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Horse IgG, Fc specific.

Reactivity: Equine

Cross Reactivity:
The antiserum does not cross-react with any other component of the Horse immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.