IgG (Fc specific) polyclonal, anti-human

Quantity: 100 µl

Background:
The reactivity of the antiserum is restricted to the Fc part of IgG. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in human serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgG from serum or plasma.

Isotype: Goat Polyclonal

Source: Highly purified Fc fragment of normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Human IgG, Fc specific

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with the following results:Bovine - Dog - Horse - pigeon - Sheep - Cat - Guinea Pig - Monkey ++ Rabbit - Swine - Chicken - Hamster - Mouse - Rat - Turkey -Three Monkey sera were tested. A reaction with Rhesus Monkey, Cynomolgus and Baboon have been found.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.