IgG (Fc specific) polyclonal FITC, anti-human

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments. In direct staining of cytoplasmic IgG in fixed human cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of human in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Isotype: Swine Polyclonal

Source: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: FITC-coupled purified hyperimmune swine IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since IgG of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine - Cat - Chicken - Dog ﾱ Duck - Goat - Guinea Pig ﾱ Hamster ﾱ Horse - Monkey + Mouse - pigeon - Rabbit ﾱ Rat - Sheep - Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques. In ELISA a faint reaction with Dog and Rat has been observed.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.