IgG (heavy and light chains) polyclonal, anti-caprine

Quantity: 1 ml

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against goat serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Isotype: Donkey Polyclonal

Source: Purified normal IgG isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune donkey IgG, in phosphate buffered saline (PBS, pH 7.2).

Specificity:
Purified IgG fraction of polyclonal Donkey anti-Serum to Goat IgG, heavy and light chains

Reactivity: Caprine

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results:bovine ++ guinea pig ﾱ mouse ﾱ rat ﾱ cat ﾱ hamster - monkey + sheep ++ chicken - horse - pigeon - swine - dog ﾱ human + rabbit ﾱ turkey -A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.