IgG (heavy and light chains) polyclonal, anti-chicken

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal chicken serum; to prepare an immunoadsorbent for the production of Ig-free chicken serum, plasma or non- Ig preparations.

Isotype: Rabbit Polyclonal

Source: Purified normal IgG isolated from pooled chicken serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: chicken

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The degree of crossreactivity depends in addition to the phylogenetic relationship also on the concentRation of the reactants and the sensitivity of the assay format. Cross-reactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction with pigeon IgG has been observed.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.