IgG (heavy and light chains) polyclonal, anti-equine

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses IgG1 and IgG2. Reactivity with other subclasses of horse IgG has not been tested. The antiserum reacts with both Fc and Fab portions of polyclonal IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against horse serum usually a single characteristic precipitin line is observed, representing primarily IgG2. If the level of IgG1, also known as IgG(T), in the serum sample approaches the level of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in horse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal horse serum; to prepare an immunoadsorbent for the production of Ig-free horse serum, plasma or non-Ig preparations. It has not been tested for use in nephelometry, ELISA or immunohistology, but this does not exclude such use if proper controls are included.

Isotype: Swine Polyclonal

Source: Purified normal IgG isolated from pooled horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: Equine

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.