IgG (heavy and light chains) polyclonal, anti-guinea pig

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against guinea pig serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal guinea pig serum; to prepare an immunoadsorbent for the production of Ig-free guinea pig serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

Isotype: Rabbit Polyclonal

Source: Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: guinea pig

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine ﾱ Cat ﾱ Chicken - Dog ﾱ Goat ﾱ Hamster + Horse ﾱ Human ﾱ Mouse ﾱ Monkey ﾱ pigeon ﾱ Rat + Sheep - Swine - Turkey -

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.