IgG (heavy and light chains) polyclonal, anti-human

Quantity: 1 ml

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

Isotype: Swine Polyclonal

Source: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune swine IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine - Cat - Chicken - Dog - Duck - Goat - Guinea Pig - Hamster - Horse - Mouse - Monkey + pigeon - Rabbit - Rat - Sheep - Turkey -

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.