IgG (heavy and light chains) polyclonal FITC, anti-rabbit

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:20 and 1:80.

Isotype: Goat Polyclonal

Source: Purified normal IgG isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: FITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2).

Specificity:
Fluorescein isothiocyanate–conjugated IgG fraction of polyclonal Goat anti-Serum to Rabbit IgG, heavy and light chains.

Reactivity: rabbit

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results:Bovine – Goat - Mouse � Sheep - Chicken -Guinea Pig � Monkey � Swine � Dog � Horse � Rat �

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.