IgG IgA IgM (Fc specific) polyclonal, anti-human

Quantity: 1 ml

Background:
The defined antibody specificity is directed to the major isotypes of the human immunoglobulin system, including antibody to common determinants and to class specific determinants as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. The cytochemical grade of GAHu/Ig(Fc)/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting.This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.

Isotype: Goat Polyclonal

Source: Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Purified IgG fraction of polyclonal Goat anti-Serum to Human immunoglobulins IgG, IgA, IgM, Fc specific

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.