IgG IgA IgM (Fc specific) polyclonal TRITC, anti-human

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the human immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:10 and 1:40.

Isotype: Goat Polyclonal

Source: Purified immunoglobulin fractions containing IgG, IgA and IgM isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: TRITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Tetramethylrhodamine isothiocyanate–conjugated IgG fraction of polyclonal Goat anti-Serum to Human IgG, IgA and IgM, Fc specific

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.