IgG IgA IgM (heavy and light chains) polyclonal, anti-monkey

Quantity: 1 ml

Background:
The defined antibody specificity is directed to the major isotypes of the monkey immunoglobulin system and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.

Isotype: Rabbit Polyclonal

Source: Highly purified IgG, IgA and IgM isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: Monkey

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The antiserum may also react with other species as has been observed for Chimpanzee and man.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.