IgG IgA IgM (heavy and light chains) polyclonal, anti-rabbit

Quantity: 1 ml

Background:
The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rabbit plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting.This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000.

Isotype: Goat Polyclonal

Source: Highly purified IgG and pools of homogenous IgA and IgM isolated from rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Purified IgG fraction of polyclonal Goat anti-Serum to Rabbit immunoglobulins IgG, IgA, IgM, heavy and light chains.

Reactivity: rabbit

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This ntiSerum fraction has not been tested for cross-reactivity.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.