IgG IgA IgM (heavy and light chains) polyclonal FITC, anti-rabbit

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rabbit plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Isotype: Goat Polyclonal

Source: Purified polyclonal rabbit IgG, and IgA and IgM containing factions isolated from rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: FITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2).

Specificity:
Fluorescein isothiocyanate–conjugated IgG fraction of polyclonal Goat anti-Serum to Rabbit IgG, IgA and IgM, heavy and light chains.

Reactivity: rabbit

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.