IgG1 IgG2a IgG2b IgG2c (Fc specific) polyclonal TRITC, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum containsantibodies to subclass-specific determinants as well as to determinants shared by two or more subclassesof IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserumconcentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained withIgA, IgM and IgG/Fab fragments or any other serum protein. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:10 and 1:30.

Isotype: Goat Polyclonal

Source: Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: TRITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat anti-Serum to Rat IgG, Fc specific.

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.