IgG1 IgG2a IgG2b IgG2c heavy and light chain polyclonal, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. Itincludes a certain degree of reactivity with other immunoglobulins via the common Fab portion.It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis anddouble radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal rat serum; to prepare an immunoadsorbent for the production of Ig-free rat serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

Isotype: Goat Polyclonal

Source: Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Rat IgG, heavy and light chains.

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results:Bovine - Dog - Horse - Monkey - Sheep - Cat - Guinea Pig ﾱ Human -pigeon - Swine - Chicken - Hamster - Mouse - Rabbit - Turkey -

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.