IgG1 IgG2a IgG2b IgG2c heavy and light chain polyclonal, anti-rat

Quantity: 1 ml

Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.

Isotype: Rabbit Polyclonal

Source: Purified normal IgG isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine + Cat ﾱ Chicken - Dog ﾱ Duck - ferret + Goat + Guinea Pig + Hamster + Horse + Human ﾱ Mouse + Monkey + pigeon - Sheep + Swine + Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.