IgG1 IgG2a IgG2b IgG2c IgA IgM (Fc specific) polyclonal, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunits of the major immunoglobulins. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rat serum.This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

Isotype: Goat Polyclonal

Source: Purified polyclonal IgG and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Rat immunoglobulins, IgG, IgA and IgM, Fc specific.

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse has been observed

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.