IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non and Mouse) polyclonal TRITC, anti-rat

Quantity: 200 µl

Background:
he reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Isotype: Goat Polyclonal

Source: Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: TRITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Tetramethylrhodamine isothiocyanate–conjugated IgG fraction of polyclonal Goat anti-Serum to Rat IgG, IgA and IgM, heavy and light chains, adsorbed for Human and Mouse cross-reactivity.

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. If the product is to be used in the presence of material originating from an other species than Human and Mouse, prior screening for cross-reactivity with that material is essential and additional adsorption of the reagent may be required to ensure intra-assay specificity.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.